Primer information
This is a sheet of info for all of the primers I have used
B7sym15
| Name | B7Sym15(forward) | |
| Sequence | CTCACCTTGAAATCAGTAGCCA | |
| Annealing Temp | 59 | |
| Genome | Nuclear | |
| Aprox. length | ||
| Reference | Pettay DT, LaJeunesse TC (2007) | |
| Name | B7Sym15(reverse) | |
| Sequence | CGTAGCTTCTGAAGGTACGACAC | |
| Annealing Temp | 59 | |
| Genome | Nuclear | |
| Reference | Pettay DT, LaJeunesse TC (2007) | |
| Aprox. length | 300-400bp |
CA4
| Name | CA4.86R | |
| Sequence | GCCTTCAATGCAATCACCTT | |
| Annealing Temp | 50 | |
| Genome | Nuclear | |
| Reference | Santos SR, Shearer TL, Hannes AR, Coffroth MA (2004) | |
| Name | CA4.86L | |
| Sequence | GGAATTGGCCATCCCTCTAT | |
| Annealing Temp | 50 | |
| Genome | Nuclear | |
| Reference | Santos SR, Shearer TL, Hannes AR, Coffroth MA (2004) | |
| Aprox. length | 200-350bp |
CA6
| Name | CA6.38R | |
| Sequence | CAAAGAATATTCGGGGGTCA | |
| Annealing Temp | 56 | |
| Genome | Nuclear | |
| Reference | Santos, S.R., Guiterrez-Rodriguez, C., Lasker, H.R., Coffroth, M.A., 2003. | |
| Name | CA6.38L | |
| Sequence | AGTTGATACGCCGGATGTGT | |
| Annealing Temp | 56 | |
| Genome | Nuclear | |
| Reference | Santos, S.R., Guiterrez-Rodriguez, C., Lasker, H.R., Coffroth, M.A., 2003. | |
| Aprox. length | 100-200 |
cp23S-rDNA (24F)
| Name | 24 F | |
| Sequence | GGTAGCAAATTTCCTTGTCG | |
| Annealing Temp | 55 | |
| Genome | Chloroplast | |
| Reference | New Primer | |
| Name | 454 R | |
| Sequence | CTTGTATCGCTTTGTTCACC | |
| Annealing Temp | 55 | |
| Genome | Chloroplast | |
| Reference | New Primer | |
| Aprox. length | 300-450bp |
cp23S-rDNA (23S1M13)
| Name | 23S1M13 | |
| Sequence | CACGACGTTGTAAAA CGACGG CTGTAACTATAACGGTCC | |
| Annealing Temp | 55 | |
| Genome | Chloroplast | |
| Reference | Harris, E.H., Boyton, I.E., Gillham, N.W., 1994. | |
| Name | 23S2M13 | |
| Sequence | GGATAACAATTTCACACAGGCCATCGTATTGA ACCCAGC | |
| Annealing Temp | 55 | |
| Genome | Chloroplast | |
| Reference | Harris, E.H., Boyton, I.E., Gillham, N.W., 1994. | |
| Aprox. length | 800-1200bp |
ITS2
| Name | ITSintfor2 | |
| Sequence | GAATTGCAGA ACTCCGTG | |
| Annealing Temp | 52 | |
| Genome | Nuclear | |
| Reference | LaJeunesse TC, Trench RK (2000) | |
| Name | ITS2_Reverse | |
| Sequence | GGGATCCATATGCTTAAGTTCAGCGGGT | |
| Annealing Temp | 52 | |
| Genome | Nuclear | |
| Reference | LaJeunesse TC, Trench RK (2000) | |
| Aprox. length | 300-450bp |
ITS2_Coral (28S)
| Name | 5.8S-436 | |
| Sequence | AGCATGTCTGTCTGAGTGTTGG | |
| Annealing Temp | 56 | |
| Genome | ITS2 | |
| Reference | Grajales A, Aguilar C, Sanchez J (2007) | |
| Name | 28S-663 | |
| Sequence | AGCATGTCTGTCTGAGTGTTGG | |
| Annealing Temp | 56 | |
| Genome | ITS2 | |
| Reference | Grajales A, Aguilar C, Sanchez J (2007) | |
| Aprox. length | 200-350bp |
PsbA-Coding (IA2)
| Name | IA2F | |
| Sequence | GGATGGGTAGAGAATGGGAATTCAG | |
| Annealing Temp | 52 | |
| Genome | Plastid | |
| Reference | Barbrook AC, Visram S, Douglas AE, Howe CJ (2006) | |
| Name | IA2R | |
| Sequence | CGAGAGTTATTRAAGGAACCATATTG | |
| Annealing Temp | 52 | |
| Genome | Plastid | |
| Reference | Barbrook AC, Visram S, Douglas AE, Howe CJ (2006) | |
| Aprox. length | 400-700bp |
PsbA-NonCoding
| Name | 1,788F | |
| Sequence | GAAAGAAATGCTCATAATTTCCC | |
| Annealing Temp | 54 | |
| Genome | Plastid | |
| Reference | New Primer | |
| Name | 2218R | |
| Sequence | TGCAGATCTCACACCAATGC | |
| Annealing Temp | 58 | |
| Genome | Plastid | |
| Reference | New Primer | |
| Name | 2282R | |
| Sequence | TTTCCATTGCGAATTTACGG | |
| Annealing Temp | 55 | |
| Genome | Plastid | |
| Reference | New Primer | |
| Name | 2282R | |
| Sequence | TTTATTTTGCACTTCAGGCC | |
| Annealing Temp | 54 | |
| Genome | Plastid | |
| Reference | New Primer | |
| Aprox. length | 200-600bp? |
ITS1
| Name | ITS1 | |
| Sequence | GGGATCCGTTTCCGTAGGTGAACCTGC | |
| Annealing Temp | 59 | |
| Genome | Plastid | |
| Reference | LaJeunesse T.C., Reyes-Bonilla H., Warner M.E., Wills M., Schmidt G.W. 2008. | |
| Name | ITS1intrev2 | |
| Sequence | TTCACGGAGTTCTGCAAT | |
| Annealing Temp | 59 | |
| Genome | Plastid | |
| Reference | LaJeunesse T.C., Reyes-Bonilla H., Warner M.E., Wills M., Schmidt G.W. 2008. | |
| Aprox. length | 300-450bp |
Written on July 29, 2019