E1 E2 E3 E4 E5 P1 P2 P3 P4 P5

Procedure

8119.2 microleters of buffer and 40.8 microleters of quant-it reagent were mixed into a master mixed for 40 reactions (should have been 42) + a 2% error

190 microleters of the master mix was mixed with 10 microleters of standard 1 and standard 2 199 microleters of the master mix was mixed with 1 microleter of each post-PCR DNA sample

The samples were run through the Qubit on the dsDNA - Broad Range setting. The nanograms of DNA per microleter was determinded

Sample	24F #1	24F #2	28S #1	28S #2	IA2 #1	IA2 #2	ITS1 #1	ITS1 #2
E1	45.8 ng/μl	43.0	86.0	83.0	94.0	91.0	31.6	31.0
E2	49.2 ng/μl	48.4	86.0	85.2	105	101	30.2	29.6
E3	53.2 ng/μl	52.6	80.8	78.8	104	103	21.4	21.0
E4	55.2 ng/μl	54.6	82.4	80.8	105	96.0	31.2	30.6
E5	53.2 ng/μl	52.8	80.0	79.2	107	105	29.2	28.8
P1	39.2 ng/μl	38.8	45.2	44.0	19.0	18.6	27.0	26.6
P2	18.2 ng/μl	18.0	26.2	25.8	12.9	12.7	14.7	14.5
P3	39.6 ng/μl	39.4	45.0	44.4	13.2	13.0	10.2	10.1
P4	45.0 ng/μl	44.8	48.0	47.0	17.8	17.5	24.4	24.0
P5	46.2 ng/μl	46.0	8.44	8.20	9.62	9.46	20.8	20.4
Standard 1	165.88 ng/μl
Standard 2	17385.21 ng/μl

All of these are in ng/μl

For 24F E1-5 was taken from the 7-15-19 tube. P1-5 was taken from the 7-16-19 tube

For 28S E1-5 was taken from the 7-17-19 tube. P1-5 was taken from the 7-22-19

For IA2 E1-5 was taken from 7-23-19. P1 and P3-5 was taken from 7-24-19 P2 was from 7-29-19.

For ITS1 E1 E2 E3 and E5 was taken from 6-18-19 and E4 was taken from 6-19-19. P1-5 was taken from 6-19-19.

Notes

• P5 for 28S was weak in the PCR and came out as ~8.3

This Data will be used to create aloquates to send to be sequenced.