Learning DNA extractions (2/2)

Procedure

Took samples out of thermomixer after 28 hours Vortexted samples after observing the tissue had been dissolved

added 200 microliters Al buffer to each and Vortexted added 200 microliters of 100% ethonol

transfered samples to spin columns centrifuged for 1 minute at 8000 rpm

transfered spin columns to new collection tubes added 500 microliters of buffer AW1 centrifuged for 1 minute at 8000 rpm

transfered spin columns to new collection tubes added 500 microliters of buffer AW2 centrifuged for 3 minutes at 14000 rpm

transfered spin columns to eppendorf tubes added 50 microliters of buffer AE (warmed to 56°C) let liquid incubate for 5 minutes at room temperature centrifuged for 1 minute at 8000 rpm added 30 microliters of buffer AE (warmed to 56°C) incubated for 5 minutes at room temperature centrifuged for 1 minute at 8000 rpm

labeled as E1, E5, E18, E20 placed in box labeled “DNA Extractons Genus: Eunicia” placed box in -20° standup freezer

• This protocol modified from DNeasy Blood and Tissue Handbook