Learning DNA extractions (1/2)

Procedure

added 180 microliters ATL to four 1.5ml eppendorf tubes Labeled the tubes E1, E5, E18, E20

Took original coral samples of sample E1, E5, E18, E20 from -20° freezer

• E1 was running low on DNA
• E5’s Dna looked brown while aloquating
• E18 and E20 were to have more samples to practice with

For each coral: took coral out of tube placed on glass slide cut small piece of tissue off placed in the corresponding eppendorf tubes

added 20 microliters of Protinase k to each

samples were placed in Thermomixer set to 56°C and 600rpm left to mix overnight

• This protocol modified from DNeasy Blood and Tissue Handbook